PCR method introduction:

Nurse unpacking box with Covid-19 PCR kit in hospital warehouse, conceptual image

Fluorescence quantitative PCR (qPCR) : based on conventional PCR, “probe for mycoplasma specific conserved sequence” is labeled with fluorescence to make PCR amplified products with fluorescence, and the change of fluorescence signal and supporting software are used to carry out real-time monitoring of DNA amplification reaction, referred to as qPCR.

Commonly used culture vessels and cleaning and disinfection

Petri dish with a blood sample in the hands of a scientist in the laboratory.

Cell culture requires a large number of consumable items, such as glassware, metal ware, plastic, rubber products, cloth, paper, etc. Therefore, it is necessary to master the knowledge of cleaning and disinfection and learn the methods of cleaning and disinfection to engage in cell culture.

Operation method and precautions of cell cryopreservation

Cryopreservation. Biobanking technician in protective gear working with biological samples

When cells cold below zero, organelles dehydration, soluble substance concentration in the cell increases, and in the formation of ice crystals in the cell, the size of the ice crystals have an effect on cell is different. So how do we solve these problems?

Depth of immersion of pipette tips

Kelabscience 1250ul Filter Tips Boxed, Angled Front View

Laboratory pipette selection range:

Overall, the usable range of the pipette is 10-100% of the maximum range of the pipette.

The best recommendation based on operating experience is: The optimal range of the pipette is 35-100% of the maximum range of the pipette.

How To Check Pipette Accuracy Quickly

Clinical laboratory in process of chemical analysis. Collection of probe using automatic pipette.

This article provides readers with common methods for determining whether a pipette is leaking, and discusses different ways to deal with each type of situation.