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Deep-well plate for lab use – PCR consumables

Table of Contents

technician in laboratory

What is the deep well plate?

On the basis of the apparent size of ordinary microporous plates (mainly 96-384 plates), the length and width should be kept in line with SBS international standards, while increasing the depth of the holes, so as to increase the volume of each hole. And in order to adapt to its specific scope of use, on the one hand by changing the manufacturing materials (polypropylene (PP), individual use of polystyrene (PS)), on the other hand by improving the surface treatment process, and then produced into a class of laboratory plate

Deep-well plate classification

  1. According to the number of hole classification, the more common can be divided into 96-well plate, 384-well plate, 96-well plate.
  2. According to the classification of hole type, the 96-well plate can be divided into round hole type and square hole type. The 384-well plates are all square holes.
  3. According to the shape of the hole bottom, there are mainly TWO types: U type and V type.

The scope of use of deep well plate

  1. Sample storage: it can replace the conventional 1.5ml centrifugal tube for storage of samples, and placed neatly, save space, large storage capacity, and can withstand -80℃. So it’s called a storage block.
  2. Sample processing: it can be combined with high-throughput automatic liquid handling equipment and software, to achieve high-throughput operations on biological samples, such as protein precipitation, and liquid-liquid extraction. It improves the efficiency of sample processing greatly. PP material can withstand 121℃ high-temperature autoclave treatment.
  3. Sample operation: it is in common use for all kinds of automatic sampler, which can be directly in the sample storehouse of automatic sampler sampling, compared with the traditional sample vial, not only can double the number of samples is put sample storehouse, it also implements the samples on 96-well plates after processing sample directly, eliminating some tedious work, such as the drain samples back and forth, put the sample, cover with lid, intubation, washing bottles, etc.

The difference between deep-well plate and PCR plate

  1. the function of deep-well plate is nucleic acid extraction and preservation, PP material (each factory has corresponding adjustment and parameters of its own PP material), the domestic is mainly round bottom, the foreign is mainly pointed bottom.
  2. PCR plate is used to replicate the sample. It has a flat bottom, low height (small capacity), high transparency, and mainly uses PC and PS materials, which are relatively brittle.

Three kinds of sealing

  1. self-adhesive, which is mostly used in school laboratories, the interval is short, price is low.
  2. low transparency silica, which is mostly used in professional laboratories, is expensive.
  3. heat sealing film (160-170 degrees), which is used for long-distance transportation, the material is PP mixed aluminum material, the price is moderate.

The sterilization ways

  1. ray sterilization (gamma), will lead to the overall color yellow but does not affect the use.
  2. gas sterilization (epoxy ring).
  3. electronic sterilization (ray), the nozzle is generally this kind of sterilization.

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