PCR Sealing Film Guide for Reliable qPCR Results
Introduction – Why PCR Sealing Films Matter in qPCR Anyone who has spent hours troubleshooting inconsistent Ct values knows that precision in qPCR is about more than reagents or instruments. The small details—like how well each reaction well is sealed—often decide whether an assay succeeds or drifts into noise. In the rush of daily lab […]
Spin Columns for DNA & RNA Purification: Technical Performance, Applications & Selection Guide
Introduction In molecular biology, the purity of nucleic acids directly determines the reliability of downstream data. Whether researchers are running PCR cleanup, plasmid preparation, or RNA analysis, even small variations in sample purity can alter Ct values, bias sequencing reads, and reduce reproducibility. Spin columns remain one of the most trusted approaches for DNA and […]
Deep-well plate for lab use – PCR consumables
In this paper, the deep well plate is introduced from the perspectives of classification, the scope of use, sealing method, sterilization method, and the difference between the deep well plate and PPR plate, so that readers have a clear understanding of the deep well plate.
Extraction and identification of plasmid DNA
Plasmids extracted by alkali lysis are separated according to the topological differences between covalently closed circular plasmid DNA and linear chromosome DNA. Within the narrow pH range of 12.0 to 12.5, the linear DNA double helix is unwound and denatured.
PCR method introduction:
Fluorescence quantitative PCR (qPCR) : based on conventional PCR, “probe for mycoplasma specific conserved sequence” is labeled with fluorescence to make PCR amplified products with fluorescence, and the change of fluorescence signal and supporting software are used to carry out real-time monitoring of DNA amplification reaction, referred to as qPCR.