This paper introduces the principle of PCR technology and the common causes of PCR laboratory pollution in general and expounds on how to avoid the occurrence of PCR laboratory pollution from the perspectives of the layout standard of the working area, the pressure control requirements of the laboratory and the standard operation of the experimental process. Readers can have a comprehensive and intuitive understanding of the antecedents and prevention measures of PCR laboratory contamination.

In this paper, the deep well plate is introduced from the perspectives of classification, the scope of use, sealing method, sterilization method, and the difference between the deep well plate and PPR plate, so that readers have a clear understanding of the deep well plate.

There are many different types of tips to choose from market. How do you know which pipette is best for your pipettor? Never be worried, that’s what we’re doing here with pipetting filter tips. This short guide will help you understand your options so that you can choose the right pipette tip and prevent costly experimental errors and all other problems.

Plasmids extracted by alkali lysis are separated according to the topological differences between covalently closed circular plasmid DNA and linear chromosome DNA. Within the narrow pH range of 12.0 to 12.5, the linear DNA double helix is unwound and denatured.

Centrifuge is a very common laboratory separation instrument, widely used in biomedicine, petrochemical, agriculture, food hygiene and other fields.

Fluorescence quantitative PCR (qPCR) : based on conventional PCR, “probe for mycoplasma specific conserved sequence” is labeled with fluorescence to make PCR amplified products with fluorescence, and the change of fluorescence signal and supporting software are used to carry out real-time monitoring of DNA amplification reaction, referred to as qPCR.

Cell culture requires a large number of consumable items, such as glassware, metal ware, plastic, rubber products, cloth, paper, etc. Therefore, it is necessary to master the knowledge of cleaning and disinfection and learn the methods of cleaning and disinfection to engage in cell culture.